Fig. 1
From: Effect of microbial siderophores on mammalian non-malignant and malignant cell lines
HR-LC/MS of ferri-exochelin-MS: Exo-MS preparation was analyzed by injecting 5 μL volume in the column. Agilent system Q-TOF Mass Spectrometer was used. The solvent system was 95 mL of 0.1% of Trifluoroacetic acid (TFA) in water with 5 mL of 0.1% TFA in 90% Acetonitrile (ACN) for 22 min. Then, 5 mL of 0.1% of Trifluoroacetic acid (TFA) in water with 95 mL of 0.1% TFA in 90% Acetonitrile (ACN) for 9 min. This was followed by the first solvent mixture for 5 min. The flow-rate was 0.4 mL/min for 35 min with maximum pressure of 1200 bar. a High-resolution liquid chromatogram: The peak seen between 24.036 and 25.271 showed the maximum area which was used for further resolution and detection by MS. b Mass spectrometry of the peak at 24 min from (a). Peaks at 663.45 and 326.37 are due to MH+ and MH22+, respectively