Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Bakr, Riham Omar; El-Naa, Mona Mohamed; Zaghloul, Soumaya Saad; Omar, Mahmoud Mohamed

doi:10.1186/s12906-017-1561-2

BMC Complementary Medicine and Therapies

Table 1 Peak assignments and tentative identification of the major constituents in N. alba AEE by HRESI-MS/MS^a in the positive and negative modes

From: Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Peak Number	Tentatively Identified Compound	tR^b (min.)	[M-H]⁻m/z^d	Negative Ionization MS/MS^c	[M + H]⁺m/z^d	Positive Ionization MS/MS
1	HHDP^e -hexoside	1.85	481.06	301.14 463.2 275.18	483.08	465.14 437.07 309.07 263
2	Epicatechin derivative	2.94	427.09	265.15 307.14 367.23 289.19 247.1 221.15
3	Ellagitannin derivative	3.59	817.07	773 481 301.11 481.24 275.19 247.23	819.09	801.2 481.13 463.05 337.14 319.14 303.12
4	HHDP-galloyl-ellagic acid	4.12	773.09	301.17 729 471.27 275.24 247
5	Ellagic acid	4.44	301.15	257.22 229.10 185.16	303.1	275.03 257.1 247.04 229.13 165.02
6	Isorhamnetin derivative	5.38	386.96	315 343.21 271.16 255.16 189.02 161.06
7	Quercetin 3–O-acetyl hexoside	12.73	505.07	459.19 403.18 487.14 301.12 275.17 231.25 247.09 169.12	507.09	489.14 447.14 405.14 387.03 303.18 205.14187.17
8	Ellagic rhamnosyl hexoside	13.27	609.17	403.17 563.18 447.3 429.2 359.2 291.22 301.13 275.28 247.19	611.18	593.30 551.30 533.27 449.30 389.21 343.21 303.22
9	Lagerstannin A (Bis-HHDP-gluconic acid)	13.40	799.06	497.16 301.21 755.24 453.26	801.08	463.15 765.11 783.17 481.15 337.12 303.15 277.10 247.15
10	Brevifolin	13.5	247.02	203.11 bp 175.13	249.6	207.05 232.12 221.12 187.08 159.04 131.04
11	Phyllanthusiin U	13.72	924.11	301.01 622.2 604.24 905.22 451.19	926.13	908.2 303.12 277.15 703.25 606.26
12	Valoneic acid dilactone dimer	13.81	939.02	469.24 425.21 300.2	941.02	453.15 922.27 621.2 470.99 407.19
13	3,4,8,9,10-Pentahydroxydibenzo[b,d]pyran-6-one	14.13	275.02	245.05 257.08 231.1 229.2 203.08 187.18	277.03	235.23 221.17 183.17
14	Luteolin	14.34	285.04	257.16 241.13 229.2 213.23 185.2 167.24
15	Ellagitannin derivative	14.45	931.11	301.14 783.2	933.03	873.05 915
16	Methyl gallate	14.56	183.03	168.13 124.03 169.09	185.33	167.07 153.03 104.96 129.07
17	Ellagic acid hexoside	15.54	463.05	301.2 419.2 417.16 331.7 274.96 251.07 247.2	465.05	303.09 447.13
18	Catechin or epicatechin derivative	15.83	621.07	575.46 439.15 289.19 245.10 217.14
19	Orientin	16.3	447.02	403.16 233.03 427.03 359.17 277.12 357.18 329.16 327.18 315.23 301.19 287.17 189.05
20	Ellagitannin derivative	16.63	755.07	711.24 453.19 301.21 435.21 409.36 275 247.23
21	Phyllanthusiin E	17.04	291.04	247.11 203.18	293.03	247.11 275.06
22	Ellagitannin derivative	17.17	1153.09	799.17 755.21 453.16 409.24
23	Phyllanthusiin B isomer	18.05	969.09	631.25 667.22 451.15 331.27 291.07
24	Castalin derivative	18.77	967.07	631.14 451.2 571.19	969.08	950.82 499.10 579.02 481.16 453.10 355.1 337.17
25	HHDP-gluconic acid	19.21	497.06	463.20 453.24 301.07 291.18 275.18 257.25 247.23
26	Ellagic acid-galloyl hexoside	20.85	613.05	451.13 425.26 569.22 509.16
27	Phyllanthusiin B	21.17	969.09	633.26 923.18 951.26 667.26 ch301.24 451.30 275.18	971.1	953.16 651.14 633.17 615.15 481.21 453.18 303.15 277.17
28	Vescalagin or castalagin	21.28	933.06	613.20 301.15 631.2 273.14 915.21	935.07	916.31 615.34 481.12 453.13 303.19 277.18
29	Apigenin	24.66	269.03	223.01 179.07
30	Phyllanthusiin C	24.91	925.10	605.29 301.08 623.23 905.15 633.08 453.09 291.18 273.06	927.11	909.26 607.23 589.14 571.16 419.23 321.19 303.1 277.11
31	Corilagin (Galloyl HHDP hexoside)	25.14	633.07	301.14 589.22 481.29 275.27 257.15	635.08	443.11 617.23 573.16 355.11 254.15 191.12
32	Pedunculagin (Bis HHDP-hexoside)	25.77	783.07	481.17 737.33 301.11 451.11	785.08	767.15
33	Castalin	26.17	631.06	301.20 299.12 451.18 271.05	633.07	615.12
34	Phyllanthusiin C derivative	27.24	1217.12	925.56
35	Lagerstannin C (Galloyl-HHDP-gluconic acid	27.33	649.1	603.26 605.27 631.23 469.18 441.17 451.26 301.14 247.19	651.08	633.13 481.19 337.11
36	Catechin or epicatechin	28.29	289.12	245.09 217.18	291.42	247.1 272.03 235.09 219.07 191.07
37	Lagerstannin B (flavogalloyl HHDP-gluconic acid)	29.82	949.11	647.25 905.25 629.24 477.24 333.21 301.15 275.2
38	Granatin B(Galloyl-HHDP-DHHDP-hex)	30.97	951.08	605.26 649.21 301.15 631.23 907.21 952.19 497.23	953.08	277.10 935.15 633.24 615.12 321.11 303.11
39	Dehydrated tergallic C-glucoside	31.4	613.05	299.07 301.27 569.27 595.15 227.15	615.06	453.14 387.09 313.15
40	Cinnamic acid derv.	31.5	329.09	197.10 239.1 169.07	331.13	299.09 313.19 272.12 236.09 151.04
41	Phyllanthusin B derivative	32.18	981.59	969.23 933.23 613.24 481.26
42	Valoneic acid dilactone	32.47	469	425.15 301.09
43	Gallic acid derivative	32.61	198.62	169.06 153.14 125.21 117.06
44	Ellagitannin derivative	33.31	907.08	605.19 863.09 587..23 561.23 301.14 275.11	909.09	890.22 703.08 589.21 321.19 303.05 277.09
45	Digalloyl ellagic acid	33.62	605.62	561.25 247.18 291.12 203.18 453.24 435.12 229.08	607	315.05 589.27 565.23 549.25 505.27
46	Castalgin derivative	34.51	965.09	631.22 301.03	967.10	647.25 949.1 906.2 629.26 495.16 445.26 303.18 27.10
47	Chebulagic acid	34.79	953.18	935.32, 907.22 649.28 605.1 631.28 301.22 291.24	955.19	937.20 633.17 481.26 463.28 319.11 301.16 27.19
48	Ellagic acid pentoside	38.56	433.04	291.15 bp 405.17 301.18 275.23 247 229	435.15	417.19 bp 407.25 399.14 376.35 343.11 325.11 299.19 181.19
49	Ellagic acid rhamnosyl	39.07	447.02	359.17 403.2 385.11 315.25 301.13 275.18	448.98	413.09 405.24 331.08 277.1
50	Geraniin	40.40	951.07	933.10 613.20 301.13 631.15
51	Vescalagin derivative	40.8	965.09	613.2 933.19 301.09	967.10	935.17 647.22 277.12
52	Galloyl ellagic acid	41.83	453.04	301.13 438.34 291.12 247.11 169.13 273.27	455.08	437.13 394.98
53	Gallic acid	62.11	169.01	125.11

Boldface digits reflects the base peak (100% abundance)
Fragment ions are listed in order of relative abundances
^aHigh resolution liquid chromatography coupled with electrospray ionisation mass /mass spectometry
^bRetention time
^cMass/Mass spectrometry
^dMass to charge ratio
^eHexahydroxydiphenic acid

Back to article page

ISSN: 2662-7671

Contact us

Submission enquiries: bmccomplementarymedicineandtherapies@biomedcentral.com
General enquiries: ORSupport@springernature.com