Fig. 6

The inhibitory effect of CBTE on the LPS-induced phosphorylation of MAPKs in RAW264.7 cells. The levels of phosphorylation of MAPK proteins were monitored 1 h after treatment of cells with LPS (1 μg/ml) either with or without CBTE pre-treatment (i.e. 1 h before LPS). Expressions of the MAPKs protein were determined by immunoblotting using specific anti-p-p38, anti-p-ERK and anti-p-JNK antibodies. An antibody against β-actin was used to verify equal protein loading of the cell lysate (a). The relative levels of the MAPKs were measured by scanning densitometry (b c d). Values represent the mean ± S.D. of three independent experiments (significant as compared to the control, **P < 0.01, significant as compared to LPS alone, ^## P < 0.01). CBTE, Cheongsangbangpung-tang extract; MAPK, Mitogen-activated protein kinases; ERK, extracellular signal-regulated kinase; JNK, Jun N-terminal kinase