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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Anvillea garcinii extract inhibits the oxidative burst of primary human neutrophils

Fig. 2

Effect of Anv and Zyg on PKC and p47phox translocation and on p47phox phosphorylation in human neutrophils. In figures a, b and c, neutrophils were incubated for 15 mins at 37 °C either without or with 300 μg/ml of water-soluble extracts from Anv or Zygophyllum gaettulum (Zyg), followed by PMA stimulation for 8 min. Zyg has no significant effect on phagocyte O2.- generation and represents a control versus Anv. Cells were lysed and membranes were purified as described in Methods and analyzed by SDS-PAGE and Western blot with anti-p47phox (a) or anti-PKCβ (b) and anti-p22phox Ab (p22phox) antibodies. Western blots from different experiments were scanned and the intensity of p47phox and PKCβ translocation was expressed relative to the protein amount of p22phox. The cumulated data is shown in the histogram as a percentage to control (PMA alone). In figure c, immunoblotting was performed with anti-phospho-Ser328 Ab, or anti-p47phox Ab (p47phox). The cumulated data is shown in the histogram as a percentage to control (PMA alone). All the results are expressed as means ± SEM of three or more separate experiments. * P < 0.05, compared to control values

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