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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: Anvillea garcinii extract inhibits the oxidative burst of primary human neutrophils

Fig. 1

Effect of Anv on superoxide anion production by human neutrophils. Human neutrophils were incubated at 37 °C during 15 min in the presence or the absence of different concentrations of Anv (a). Then they were stimulated with either fMLF (10−6 M) or PMA (100 ng/mL) and simultaneously, superoxide production was measured using the cytochrome c reduction assay at 550 nm in a spectrophotometer for 10 min. The amount of superoxide produced is expressed as a percentage of values obtained when neutrophils were stimulated with fMLF or PMA alone. In b, we investigated if Anv extract can scavenge superoxide anion. The O2.- generation substrate xanthine and cytochrome c were incubated with different concentrations of Anv extract at 30 °C during 15 min. The enzyme xanthine oxidase was added and the amount O2.- was measured by cytochrome c reduction assay. All results are means ± SEM of three or more separate experiments. ** P < 0.01, *** P < 0.0001

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