Fig. 4

Colonic proliferation cells from the colon CAC mice models. The cell proliferation patterns in the colon tissue were assessed by using BrdU immunohistochemistry. Epithelial proliferation enhanced in colon of CAC mice model. Colon tissues were treated with anti BrDu antibody counterstained with hematoxylin and visualized under the light microscope. Cell proliferation identified by BrdU immunohistochemical staining of paraffin section. Brown-color nucleus indicates BrdU-positive cells. (A) Control group received water, (B) Group was administrated DSS 5 % only, (C), (D) and (E) mice were administrated DPE orally 125, 250 and 500 mg/kg, respectively. Red arrow: the BrdU positive cell. All images were magnified at 400x and representative four independent experiments with consistent results. (F). Number of BrdU positive cells. Results shown are mean + SD, with n = 4 replicates in each group. Results are mean ± SD. *, p < 0.05. **, p < 0.001