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Table 3 Gradient Table for Gradient Pump

From: Isolation of alpha-linolenic acid from Sutherlandia frutescens and its inhibition of Mycobacterium tuberculosis’ shikimate kinase enzyme

Time (min)

Flow (mL/min)

% HOH

% MeOH

% 0.1 % FA HOH

% CAN

0.0

5.5

0

65

35

0

2.0

5.5

0

65

35

0

15.0

5.5

0

75

12

13

25.0

5.5

0

0

0

100

40.0

5.5

0

0

0

100

44.0

5.5

0

65

35

0

50.0

5.5

0

65

35

0

  1. Column heater temperature: 60 °C
  2. PDA: Scanning 210–600 nm
  3. Collection: Hold time: 1 min, 2 min collection intervals for 20 vials
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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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