Fig. 6

PPHs regulate ROS release, NADPH oxidase, and SOD activity in MC3T3-E1 osteoblastic cells. a ROS production was analyzed in cells treated for 6 h with 400 μM H₂O₂ in the presence or absence of 100 μg/mL PPHs by the DCF-DA assay. NADPH oxidase (b) and SOD activities (c) were assessed in cells treated for 6 h with 400 μM H₂O₂ in the presence or absence of 100 μg/mL PPHs. Representative bands are shown from three independent experiments. Quantitative analysis of RNA expression was also performed. ^* p < 0.05 versus cells treated with H₂O₂ alone. PPHs, porcine placenta hydrolysates