Fig. 3

Effect of the aqueous extract from the fruits of Hancornia speciosa in zymosan-induced air pouch model. Swiss mice were treated (i.p.) with saline, aqueous extract at doses of 40, 50 or 60, rutin, chlorogenic acid at the dose of 2, 2.5 or 5 and with dexamethasone (2.0 mg/kg), thirty minutes after subcutaneous (s.c.) injection of zymosan solution (1 mg/mL) or saline (1 mL) into the animals air pouch. After six hours, the exudate was harvested from each air pouch by lavage with 2 mL of saline and leucocytes count was performed using a Neubauer chamber (a). At 6, 24 and 48 h after zymosan administration, the dose of 50 mg/kg of aqueous extract, 2.5 mg/kg of rutin and 10 mg/kg chlorogenic acid were chosen to perform the experiment of leucocytes cell migration kinetics (b) and polymorphonuclear cells migration kinetics (c). The solid column (black bar) represents the group that received saline solution (i.p.) followed by subcutaneous administration of zymosan 30 min later. Each column represents the mean of the values obtained from five animals, and the vertical lines indicate the standard errors of the mean (SEM). ^###p < 0.001 compared with the control group (Sal); ***p < 0.001, **p < 0.01 and *p < 0.05, compared with the zymosan group (Zym). Sal: saline (0.9 mg/mL); Zym: zymosan (1 mg/mL); Dx: dexamethasone (2.0 mg/kg); AE: aqueous extract (50 mg/kg); RT: rutin (2.5 mg/kg); CA: chlorogenic acid (10 mg/kg)