Table 6 Retention time, optimized signal wavelength, and regression analysis of reference flavonoids determined by HPLC-DAD analysis
Compound | Signal wavelength | Retention time (min) | Regression analysis | R2 |
|---|---|---|---|---|
Gallic acid | 257 | 4.35 | y = 3.0565x + 8.01 | 0.9998 |
Rutin | 257 | 15.91 | y = 1.7773x−48.49 | 0.9958 |
Catechin | 279 | 9.70 | y = 1.1764x + 7.96 | 0.989 |
Caffeic acid | 325 | 325 | y = 12.569x + 22.66 | 0.9905 |
Apigenin | 325 | 23.53 | y = 12.950x−52.12 | 0.9957 |
Myricetin | 368 | 18.72 | y = 2.560x−15.09 | 0.9994 |
Kaempherol | 368 | 23.59 | y = 10.967x + 125.59 | 0.9964 |
Quercetin | 368 | 21.31 | y = 4.8624x + 35.01 | 0.9993 |