Fig. 4

The effects of LASAP-C extract on melanogenesis in zebrafish. Synchronized embryos were treated with 10 and 100 μg/mL LASAP-C. The effects on zebrafish pigmentation were observed using a stereomicroscope. In blank control and 100 μg/mL LACAP-C treated zebrafish, the pigmentation was pointed as black arrowhead and shrinkage of pigment cells was marked as black arrow. Lateral view of embryos at 31 h post fertilization: a blank control, b 0.2 mM 1-phenyl-2-thiourea (PTU), c 10 μg/mL LASAP-C, and d 100 μg/mL