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Table 4 MIC of antibiotics after the association of resveratrol (4) at MIC/2 and MIC/4 against selected MDR bacteria

From: Antibacterial and antibiotic-resistance modifying activity of the extracts and compounds from Nauclea pobeguinii against Gram-negative multi-drug resistant phenotypes

Antibioticsa Concentration of 4 Bacteria strainsb, MIC of antibiotics alone and in presence of 4 (Resveratrol), FIC (in bracket ) PBSS (%)c
PA124 CM64 KP55 AG102 AG100Atet PS2636  
CHL 0 256 128 32 64 64 256  
  MIC/2 32 (0.25)S 2(0.02)S 16(0.5)S 64(1)I <2(<0.03)S 64(0.25)S 5/6 (83.3 %)
  MIC/4 32(0.25)S 4(0.03)S 16(0.5)S 64(1)I <2 (<0.03)S 64(0.25)S 5/6(83.3 %)
KAN 0 128 64 64 4 32 4  
  MIC/2 64(0.5)S <2 (<0.03)S 32(0.5)S <2 (<0.5)S <2(0.06)S 4(1)I 5/6(83.3 %)
  MIC/4 64(0.5)S <2 (<0.03)S 32(0.5)S <2 (<0.5)S <2(0.06)S 4(1)I 5/6(83.3 %)
STR 0 64 128 64 ≤2 ≤2  
  MIC/2 32(0.5)S 64(0.5)S 16(0.25)S <2 na 64(≤0.5)S <2 na 4/4 (100 %)
  MIC/4 32(0.5)S 64(0.5)S 32(0.5)S 4(>2) na 128(≤0.5)S <2 na 4/4 (100 %)
CIP 0 64 8 4 1 64 4  
  MIC/2 4(0.06)S <2(0.25)S <2(<0.5)S <0.5(<0.5)S <2(<0.03)S 8(2)I 5/6(83.3 %)
  MIC/4 16(0.25)S <2(0.25)S <2(<0.5)S <0.5(<0.5)S <2(<0.03)S <0.5(<0.13)S 6/6 (100 %)
TET 0 64 64 32 4 256 64  
  MIC/2 4(0.06)S 64(1)I 32(1)I <2(<0.5)S 256(1)I 32(0.5)S 3/6 (50 %)
  MIC/4 4(0.06)S 128(2)I 16(0.5)S <2(<0.5)S 256(1)I 16(0.25)S 4/6 (66.7 %)
  1. (−): >256 μg/mL; 0: without compound 4 (only antibiotic tested). CIP have been tested at 64 μg/ml on AG102 and PS2636; na: non-applicable; Values in bold: MIC of antibiotic alone or significant synergistic effects
  2. S Synergy, I Indifference, A Antagonism, P product, FIC fractional inhibitory concentration
  3. aAntibiotics [CHL: chloramphenicol, KAN : kanamycin, STR: streptomycin, CIP : ciprofloxacin, TET : tetracycline]
  4. bBacterial strains: Escherichia coli [ AG102, AG100Atet], Pseudomonas aeruginosa [PA124], Enterobacter aerogenes [CM64], Klebsiella pneumoniae [KP55], Providencia stuartii [PS2636]
  5. cPBSS: percentage of bacteria strain on which synergism has been observed