Fig. 2

In vitro direct cytotoxic effect of Gancao polysaccharide extracts. Decrease in cell number was observed in CT 26 cells (5 × 104 cells /ml) upon treatment with various concentration of polysaccharides (Fraction a, b and c) from 0.05 mg/ml to 0.8 mg/ml as measured by CCK-8 assay. Data expressed as mean ± SEM of three experiments. *P < 0.05, **P < 0.01 versus control