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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Antiproliferation of Cryptocarya concinna-derived cryptocaryone against oral cancer cells involving apoptosis, oxidative stress, and DNA damage

Fig. 3

Annexin V/PI-based apoptosis of CPC-treated oral cancer Ca9-22 cells and the effect of NAC pretreatment. With or without 2 mM NAC pretreatment for 1 h, Ca9-22 cells were treated with 0, 3, 6, 9, and 12 μM of CPC for 24 h. a Representative results of flow cytometry-based annexin V/PI double staining of CPC-treated Ca9-22 cells. Annexin V (+)/PI (+) and Annexin V (+)/PI (-) were calculated as the apoptosis (+) in each panel. b Quantification analysis of apoptosis for CPC-treated Ca9-22 cells in Fig. 3a. Data, mean ± SD (n = 3). For the same drug treatment of different concentrations, data marks (a and b) without overlapping by the same lower-case letter significantly differed (one-way ANOVA with Tukey HSD Post Hoc Test)

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