Fig. 4

Figure 4A shows the effect of Curcuma Longa (CL) on the severity of inflammatory reactions in rats when given orally 30 min after the induction of IBD for a total of 7 days. The images show that severe lymphatic infiltration (arrow), especially those transcending all layers of the colon is more prevalent in the colon of untreated IBD rats compared to CL-treated animals. (n = 10). 4A. 1: Non IBD Control, no CL (naive). 4A. 2: 2 Days IBD, (i) Control, no CL, (ii) 1 mg/kg CL, (iii)10 mg/kg CL, (iv)100 mg/kg CL. 4A. 3: 4-day IBD (i) control, no CL, (ii)1 mg/kg CL, (iii) 10 mg/kg CL, (iv)100 mg/kg CL. 4A. 4: 7-day IBD (i) control, no CL, (ii) 1 mg/kg CL, (iii) 10 mg/kg CL, (iv) 100 mg/kg CL. Figure 4B shows the effect of CL on the microscopy of the colon of acetic acid-induced IBD rats 3 days before the induction of IBD. IBD caused a significant (P < 0.01) increase in the severity of leucocyte infiltration (arrow). CL administration at doses of 100 mg/kg did not markedly reduce the degree of leucocyte infiltration. 4B.1(i):Control, no CL, no IBD. 4B.1(ii): 1 mg/kg CL, no IBD, (iii): 10 mg/kg CL, no IBD, 4B.1(iv): 100 mg/kg CL, no IBD. 4B.2(i): no CL + 2 days IBD control; (ii): 1 mg/kg CL, (iii): 10 mg/kg CL + 2 days IBD, (iv): 100 mg/Kg + CL 2 days IBD. 4B.3(i): no CL + 4 days IBD control; (ii):1 mg/kg CL + 4 days IBD, (iii):10 mg/kg CL + 4 days IBD, (iv):100 mg/kgCL + 4 days IBD. 4B.4(i): no CL + 7 days IBD control; (ii): 1 mg/kg CL + 7 days IBD, (iii): 10 mg/kg CL + 7 days IBD, (iv):100 mg/kgCL + 7 days IBD