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Table 1 Nucleotide sequences of primers and operating condition for PCR analysis

From: Bee venom acupuncture alleviates trimellitic anhydride-induced atopic dermatitis-like skin lesions in mice

Gene Nucleotide sequence Operating condition
GAPDH sense 5′-AACTTTGGCATTGTGGAAGG-3′ 94 °C, 30s 58 °C, 30s 72 °C, 30s, 30 cycles
antisense 5′-ACACATTGGGGGTAGGAACA-3′
IL-1β sense 5′-GGCTGTGGAGAAGCTGTGGC-3′
antisense 5′-GGGTGGGTGTGCCGTCTTTC-3′
TNF-α sense 5′-GCAGAAGAGGCACTCCCCCA-3′
antisense 5′-GATCCATGCCGTTGGCCAGG-3′
IL-4 sense 5′-TCAACCCCCAGCTAGTTGTC-3′
antisense 5′-TGTTCTTCGTTGCTGTGAGG-3′
  1. T thymine, A adenine, C cytosine, G guanine, GAPDH glyceraldehyde-3-phosphate dehydrogenase, IL interleukin, TNF tumor necrosis factor