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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: A1E reduces stemness and self-renewal in HPV 16-positive cervical cancer stem cells

Fig. 2

A1E regulates stemness in cervical carcinoma cells. a Spheroid formation assay of A1E-treated HPV 16-positive SiHa cells grown in stem cell selective media for 0, 7, and 14 days. b Spheroid formation assay of A1E-treated HPV-negative C33A cells grown in stem cell selective media for 14 days (magnification, 100×). Bar represents 50 μm. Histogram shows the average number of spheroids formed from 2000 cells. Data are presented as mean ± SEM. **P < 0.01. c Western blotting analysis of stemness markers in A1E-treated SiHa and C33A cells

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