Fig. 9

Western blot analysis of ERK1/2 expression and ELISA assay for TNF-α. ERK1/2 expression levels normalized on ERK2 display an increase in rats subjected to CIR, while administration of bioactive TBK-SE reduces levels of ERK1/2 (a). ERK2 was used as internal control. **p < 0.05 vs CIR. ELISA assay showed that serum levels of TNF-α are significantly higher in untreated CIR rats when compared with TNF-α serum levels of animals treated with bioactive TBK-SE (b). **p < 0.05 vs CIR