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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Anti-diabetic potential of Catharanthus roseus Linn. and its effect on the glucose transport gene (GLUT-2 and GLUT-4) in streptozotocin induced diabetic wistar rats

Fig. 3

Simultaneous amplification plots and standard curves of GLUT-2 & 4 mRNA by fluorescence real-time PCR. a. Plot of threshold cycle number (Ct) vs. serial dilutions (log10) of standard DNA from 101 to 108 copies/reaction tube were prepared. Reaction number increases during PCR as the amplicon copy number increases until the reaction reaches a plateau. Ct was plotted against each copy number. Ct represents the PCR cycle at which reporter signal can first be detected. b. Standard curve showing efficiency of amplification during PCR

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