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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Attenuated RANKL-induced cytotoxicity by Portulaca oleracea ethanol extract enhances RANKL-mediated osteoclastogenesis

Fig. 4

Portulaca oleracea ethanol extract (POEE) attenuates receptor activator of NF-κB ligand (RANKL)-mediated cytotoxicity. a Bone marrow-derived macrophages (BMMs) in 96-well plates were stimulated with RANKL for the indicated time. Following incubation, glucose-6-phosphate dehydrogenase (G6PD) in the culture medium was measured. The amount of released G6PD is calculated as a percentage of total G6PD, and results are presented as relative mean fold change. b BMMs were cultured under the indicated conditions. Polyadenosine 5′-diphosphate-ribose polymerase (PARP) expression and cleavage were evaluated by performing western blot analysis. Summarized data are presented as a ratio of cleaved PARP/uncleaved PARP in lower panel. β-actin was used for loading control

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