Effects of Antrodia camphorata extracts on anti-oxidation, anti-mutagenesis and protection of DNA against hydroxyl radical damage

Table 3 Mutagenesis test for A. camphorata extract against S. typhimurum TA 98 with S9

Dosage (mg/plate)	His⁺ revertants (mutagenicity index)^a
Dosage (mg/plate)	without S9	with S9
Spontaneous revertants ^b	165 ± 10 (1.00)	198 ± 20 (1.00)
1	167 ± 9 (1.01)	186 ± 7 (0.93)
0.5	173 ± 9 (1.04)	197 ± 11 (0.99)
0.25	178 ± 10 (1.07)	202 ± 8 (1.02)
0.125	180 ± 6 (1.09)	203 ± 6 (1.02)
0.0625	173 ± 3 (1.04)	204 ± 12 (1.03)

100 μl S. typhimurium TA98 was mixed with 100 μl sample and 500 μl phosphate buffer, and incubated at 37 °C for 20 min, after that the population of S. typhimurium TA 98 was calculated
^aEach value is expressed as mean ± SD (n = 3). Mutagenicity index = induced revertants per plate/spontaneous revertants per plate
^bThe number of spontaneous revertants was determined without water extracts of A. camphorata. Values in parentheses are mutagenicity index by using spontaneous revertants as 1

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