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Table 2 The unpredictable chronic mild stress protocol

From: The effect of Piper laetispicum extract (EAE-P) during chronic unpredictable mild stress based on interrelationship of inflammatory cytokines, apoptosis cytokines and neurotrophin in the hippocampus

1

Light during the night

15

24 h of damp sawdust

2

5 min swimming at 4 °C

16

15 min Shaker rocking 1 time/second

3

24 h of food deprivation

17

Light during the night

4

24 h of water deprivation

18

24 h of water deprivation

5

Light during the night

19

1 min clipping tail

6

15 min shaker rocking 1 time/second

20

2 h of restraint

7

24 h of food deprivation

21

5 min swimming at 45 °C

8

1 min clipping tail

22

2 h of restraint

9

2 h of restraint

23

1 min clipping tail

10

5 min swimming at 45 °C

24

24 h of damp sawdust

11

5 min swimming at 4 °C

25

24 h of water deprivation

12

5 min swimming at 45 °C

26

5 min swimming at 4 °C

13

15 min shaker rocking 1 time/second

27

24 h of damp sawdust

14

24 h of food deprivation

28

5 min swimming at 4 °C