Table 1 Primer sequences used in this study
Genes | Forward primer | Reveres primer | Tm (C°) |
|---|---|---|---|
ACC | GAAGTCAGAGCCACGGCACA | GGCAATCTCAGTTCAAGCCAGTC | 62 |
FAS | AGCACTGCCTTCGGTTCAGTC | AAGAGCTGTGGAGGCCACTTG | 62 |
SREBP1c | CTCCGGCCACAAGGTACACA | GAGGCCCTAAGGGTTGACACAG | 60 |
HSL | CAAGTGTGTCAGCGCCTATGCT | GGGCTTTCTGGTCTGAGTTGGA | 64 |
PPARγ | CTTGTGAAGGATGCAAGGGT | ATACAAATGCTTTGCCAGGG | 62 |
CPT-1a | GGAATGAAATTCCCACTGTCTGTC | CAGTTCAGCCATCGCTGTTGTA | 60 |
HADHα | ACTTCCAAAGACACCAGTGCTTCA | GGCGCAAGACACCTGGTAGTATAGA | 60 |
LCAD | CCAGCTGCATGAAGCGAAAC | GCTGAACTCTGGCATCCACATAA | 60 |
UCP2 | TGTGCCCTTACCATGCTCCA | AGGGCTCGTTTCAGCTGCTC | 60 |
CYP2E1 | ATGTCTGCCCTCGGAGTGA | GATGTCCTTCCAGGTAGGTCC | 58 |
CYP4A11 | AGGAGCTCCAACAGGACCAG | CCTGATGGGTGAAGGCACAC | 68 |
PPARα | TCCTGAGCCATGCAGAATTTAC | AGTCTAAGGCCTCGCTGGTG | 60 |
TNFα | GACAAGCCTGTAGCCCATGTTGTA | CAGCCTTGGCCCTTGAAGA | 60 |
IL-6 | GTGGAGATTGTTGCCATCAACG | CAGTGGATGCAGGGATGATGTTCTG | 60 |
MCP-1 | AGCAGCAAGTGTCCCAAAGA | GGTGGTCCATGGAATCCTGA | 60 |
STAT3 | GGAATCCCGTGGGTTGCTTAC | TTGAATGCAGTGGCCAGGAC | 60 |
SOCS3 | CAGGAATGTAGCAGCGATGGAA | CCTGTCCAGCCCAATACCTGA | 60 |
GAPDH | TGAACGGGAAGCTCACTGG | TCCACCACCCTGTTGCTGTA | 58–64 |