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Table 5 The values of melting temperature (°C) with standard deviations gaining form high resolution melting (HRM) analysis using matK, rbcL, rpoC and trnL primers of T. laurifolia and C. spectabilis species

From: Fast and reliable detection of toxic Crotalaria spectabilis Roth. in Thunbergia laurifolia Lindl. herbal products using DNA barcoding coupled with HRM analysis

Species Abbreviation Tm (oC)
mat K rbc L rpo C trn L
T. laurifolia T1 - 82.5 ± 0.07 80.2 ± 0.12 78.8 ± 0.07
T. laurifolia T2 - 82.4 ± 0.14 80.1 ± 0.15 78.9 ± 0.07
T. laurifolia T3 77.9 ± 0.35 82.3 ± 0.14 80.2 ± 0.00 78.8 ± 0.00
T. laurifolia T4 - 82.4 ± 0.07 80.2 ± 0.07 78.8 ± 0.14
C. spectabilis C1 75.6 ± 0.21 81.6 ± 0.00 80.9 ± 0.06 78.4 ± 0.14
C. spectabilis C2 75.3 ± 0.07 81.6 ± 0.07 80.9 ± 0.06 -
C. spectabilis C3 75.4 ± 0.21 81.5 ± 0.00 80.8 ± 0.00 78.2 ± 0.00
  1. (-) No amplicons were generated