Figure 5
Visualization of amyloid aggregates by laser-scanning microscopy (LSM). Images were taken after overnight of aggregation at 37°C with a concentration of 50 μM unlabeled Aβ1–42(A) and 5 nM TAMRA-Aβ1–42 + 20 μM unlabeled Aβ1–42(C). Aggregates of (50 μM unlabeled) Aβ1–42 displayed characteristic green fluorescence after their binding with ThT. TAMRA-Aβ1–42 itself exhibited orange fluorescence. Asiaticoside-incubated samples had a lower number and smaller green (ThT) (B) and orange (TAMRA-Aβ1–42) (D) fluorescent areas than those of the controls, suggesting that Aβ1–42 fibrillation was inhibited by asiaticoside.