Figure 3

SHME attenuates H ₂ O ₂ -induced apoptosis and ROS generation in C2C12 cells. C2C12 cells were pretreated with 300 μg/ml SHME or 5 mM NAC for 1 h and then stimulated with and without 1 mM H₂O₂ for 6 h. (A) To quantify the degree of apoptosis, media were discarded and the cells were evaluated for sub-G1 DNA content using a flow cytometer. (B) To monitor ROS production, the cells were incubated at 37°C in the dark for 20 min with new culture media containing 10 μM DCF-DA. ROS generation was measured using a flow cytometer. Data are presented as the mean ± SEM obtained from three independent experiments (*P < 0.05, compared with the control group; ^#P < 0.05, compared with the H₂O₂-treated group).