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Figure 3 | BMC Complementary and Alternative Medicine

Figure 3

From: Sargassum horneri methanol extract rescues C2C12 murine skeletal muscle cells from oxidative stress-induced cytotoxicity through Nrf2-mediated upregulation of heme oxygenase-1

Figure 3

SHME attenuates H 2 O 2 -induced apoptosis and ROS generation in C2C12 cells. C2C12 cells were pretreated with 300 μg/ml SHME or 5 mM NAC for 1 h and then stimulated with and without 1 mM H2O2 for 6 h. (A) To quantify the degree of apoptosis, media were discarded and the cells were evaluated for sub-G1 DNA content using a flow cytometer. (B) To monitor ROS production, the cells were incubated at 37°C in the dark for 20 min with new culture media containing 10 μM DCF-DA. ROS generation was measured using a flow cytometer. Data are presented as the mean ± SEM obtained from three independent experiments (*P < 0.05, compared with the control group; #P < 0.05, compared with the H2O2-treated group).

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