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Figure 7 | BMC Complementary and Alternative Medicine

Figure 7

From: Inhibition of HIV-1 entry by extracts derived from traditional Chinese medicinal herbal plants

Figure 7

Effects of the extracts on primary HIV-1 isolate 89.6. A. U87.CD4.CXCR4 and U87.CD4.CCR5 cells were first treated with 10 μg/ml extracts and then infected with HIV-Luc viruses pseudotyped with 89.6 envelope. Infection of HIV-Luc viruses without an envelope was included as the mock infection control. B. HIV-Luc viruses pseudotyped 89.6 envelope were first incubated with 10 μg/ml extracts and then used to infect U87.CD4.CXCR4 and U87.CD4.CCR5 cells. Infection with heat-inactivated HIV-Luc/89.6 viruses (Δ Virus) was included as the control. C. U87.CD4.CXCR4 and U87.CD4.CCR5 were first infected with HIV-Luc viruses pseudotyped with 89.6 envelope and then treated with 10 μg/ml extracts or 5 μM AZT. A-C: open bar for U87.CD4.CXCR4 cells; closed bar for U87.CD4.CCR5 cells. D. HIV-Luc viruses pseudotyped with 89.6 envelope were directly treated with 10 μg/ml extracts or 5 μM AZT-TP, the RT activity was determined. The data were mean ± SEM of triplicate experiments.

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