In-vitro and in-vivo validation of ethnopharmacological uses of methanol extract of Isodon rugosus Wall. ex Benth. (Lamiaceae)

Table 1 Inhibitory activity of Ir.Cr on cholinesterases and lipoxygenase enzymatic activities in comparison to respective standards

Enzymatic activity	Test material	Conc./well	% Inhibition	IC₅₀
Acetylcholinesterase	Ir.Cr	0.5 mg/ml	41.66 ± 0.3	0.6 ± 0.04 mg/ml
Acetylcholinesterase	Eserine*	0.25 mM	91.29 ± 1.17	0.14 ± 0.001 mM
Butyrylcholinesaterase	Ir.Cr	0.5 mg/ml	33.78 ± 0.5	0.74 ± 0.02 mg/ml
Butyrylcholinesaterase	Eserine	0.25 mM	82.82 ± 1.09	0.15 ± 0.02 mM
Lipoxygenase	Ir.Cr	0.5 mg/ml	36.65 ± 2.8	0.68 ± 0.07 mg/ml
Lipoxygenase	Baicalin**	0.25 mM	93.79 ± 1.38	0.13 ± 0.015 mM

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