Figure 4

Zyflamend and its effects on cellular p21 expression as determined by immunofluorescent imaging using CWR22Rv1 cells. Cells pretreated with 200 μg/ml Zyflamend for 24 hours (right) were compared with control cells (left). Nuclei are shown in blue. Mean p21 (green) abundance per cell was calculated [(total p21 fluorescence)/(nuclei count)]. To assess nuclear accumulation, p21 fluorescence was also measured within discrete nuclear regions (defined using a DAPI intensity threshold). Data are presented as the mean ± SD in relative fluorescence units. Representative images feature magnified inlays.* p < 0.001, n = 4; ** p < 0.001, n = 112.