Figure 4
Both VitC pre- and post-treatment relieved histological damages through HO-1 induction. A) representative light microscopy images of the kidney (a, d, g, j, m, p, s, scale bar: 200 μm), liver (b, e, h, k, n, q, t, scale bar: 100 μm), and lung (c, f, I, l, o, r, u, scale bar: 200 μm), which were stained with hematoxylin and eosin. B) histological damage score of the kidney (a), liver (b), and lung (c). Tissue samples were collected at 12 hours after the HS operation. Data represents the mean ± SEM, n = 6. *p < 0.05 vs. the Sham; **p < 0.05 vs. the HS; ***p < 0.05 vs. the HSV-pre; ****p < 0.05 vs. the HSV-post; $ p > 0.05 vs. the Sham. Sham: the rats which received NS and underwent sham operation. ShamV: the rats which received VitC and underwent sham operation. HS: the rats which underwent HS operation and received NS. HSV-pre: the rats which received VitC at 6 hours before HS operation. HSV-preZ: some HSV-pre rats further received ZnPP. HSV-post: the rats which underwent HS operation and received VitC immediately. HSV-postZ: some HSV-post rats further received ZnPP.