Figure 7From: Earthworm (Pheretima aspergillum) extract stimulates osteoblast activity and inhibits osteoclast differentiation Earthworm extract inhibits the differentiation of osteoclasts. RAW 264.7 cells were seeded in 48-well plates and allowed to adhere for 1 day. Osteoclast differentiation from RAW 264.7 cells was induced with 50 ng/ml RANKL in α-MEM for 6 days. Osteoclasts were then cultured with PBS as a control or different concentrations of earthworm extract for 2 days. Osteoclast differentiation was determined by TRAP activity assay. Results are expressed as percentage of control (*p < 0.05 and **p < 0.01 vs. control).Back to article page