Confocal microscopy analysis of mitochondrial superoxide production and mitochondria. (A) MitoSOX™ Red staining followed by confocal microscopy analysis (60 × 3.5) was used to measure mitochondrial superoxide production. (B) Mitotracker® Red fluorescence stained mitochondria. Merged images of cells stained with Mitotracker® Red with 4',6-diamidino-2-phenylindole (DAPI) blue fluorescence stained cells were used to determine colocalization by using confocal microscopy (10 × 3.5). All data are reported as a percentage of control. * p < 0.05.