Figure 2

Mitochondrial respiration of PC12 cells Oxygen consumption rate (OCR) was monitored in control cells, 3-NP treated cells, and in cells that were pretreated with PCWE or PCEE followed by 3-NP treatment. (A) Cells were pretreated with PCWE at concentrations of 10, 50, and 100 μg/ml for 24 h followed by 25 μM 3-NP treatment for 3 h. (B) Cells were pretreated with PCEE at concentrations of 10, 50, and 100 μg/ml for 24 h followed by treatment with 25 μM 3-NP for 3 h. (C) Basal respiration was calculated from OCR. Vertical lines indicate time of addition of mitochondrial inhibitors A: oligomycin (1 μg/ml), B: FCCP (1 μM), or C: rotenone (1 μM). * p < 0.05, and ** p <0.01.