Figure 4

Danggui extract inhibits Ang II-induced caspase 9 (A) and caspase 3 (B) activation in H9c2 cardiomyoblast cells. H9c2 cells were treated with Ang II (5×10⁻⁷ M) for 1 hr, and then treated with Danggui extract (50, 100 μg/ml) either 1 hr before (pre-treatment) or 1 hr after (post-treatment) Ang II treatment, followed by another 23 hr of culture. Cells were harvested and extracted for Western blotting analysis. α-tubulin was employed as the internal control.