In vitro anti-Onchocerca ochengi activities of extracts and chromatographic fractions of Craterispermum laurinum and Morinda lucida

Table 3 Chromatographic profile and activity of seventeen fractions from ML _LMeOH on microfilariae motility

Eluent	Combined Fractions:code	% inhibition of microfilariae motility on day 5 concentration of extracts (μg/ml)
Hex:EtOAc	Combined Fractions:code	500	250	125	62.5	31.25	15.63	7.8	0	IC₅₀of mfs
100:0	1-13: MLM1	100	50	0	0	0	0	0	0	250
95:5	14-16: MLM2	50	0	0	0	0	0	0	0	500
95:5	17-24: MLM3	100	100	50	0	0	0	0	0	125
90:10	25-32: MLM4	50	0	0	0	0	0	0	0	500
80:20	33-40: MLM5	100	75	0	0	0	0	0	0	187.5
80:20	41-49: MLM6	50	25	0	0	0	0	0	0	500
70:30	50-54: MLM7	100	75	50	0	0	0	0	0	125
70:30	55-61: MLM8	100	100	100	50	25	0	0	0	62.5
60:40	62-65: MLM9	100	100	100	100	50	0	0	0	31.25
60:40	66-74: MLM10	100	100	100	100	100	100	50	0	7.8
50:50	75-86: MLM11	100	100	100	100	50	50	25	0	15.63
40:60	87-100:MLM12	100	100	100	50	0	0	0	0	62.5
20:80	101-109:MLM13	100	100	100	100	100	25	0	0	23.44
20:80	110-117:MLM14	100	100	100	100	50	0	0	0	31.25
CH2Cl2:MeOH
95:5	118-120:MLM15	100	100	100	50	25	25	25	0	62.5
95:5	121-128:MLM16	75	50	25	0	0	0	0	0	250
90:10	129-150:MLM17	100	50	0	0	0	0	0	0	250
80:20
70:30
60:40

MLM: M. lucida leaves. The 0 μg/ml are the negative control wells with 2% DMSO.

ISSN: 2662-7671