Figure 3

Effect of AST on permeability of BBB and ROS level. A) AST decreased permeability of BBB in EAE mice examined by Evan’s blue dye infiltration. B) AST reduced ROS release in cortices of EAE mice measured by DHE fluorescent intensity. C) AST did not increase GSH-Px activity in cortices of EAE mice. D) AST up-regulated T-SOD activity in cortices of EAE mice. E, F) AST inhibited elevated iNOS and MDA in cortices of EAE mice. The dose of AST used was 50 mg/kg/day. All data are presented as mean±standard error of mean. n = 5 in each group. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001 vs EAE group.