Figure 5
Effect of AMEAE on ROS generation in A549 cells. (A) Representative images of A549 cells treated with medium alone and 10 μg/mL of AMEAE for 24 h and stained with dihydroethidium and Hoechst 33342 dyes for 30 min. (B) Representative bar chart indicated dose-dependent elevation in ROS formation in treated A549 cells. The level of ROS was significantly elevated at 5 to 40 μg/mL concentrations. (C) Pretreatment of A549 cells with Cat and SOD for 1 h prior to the incubation with AMEAE caused a significant elevation in cell viability compared to the treatment alone with AMEAE. The data represent the means ± SEM of three independent experiments. *p < 0.05 compared with the untreated group.