The natural triterpene 3β,6β,16β-trihydroxy-lup-20(29)-ene obtained from the flowers of Combretum leprosum induces apoptosis in MCF-7 breast cancer cells

Table 2 Antiproliferative activity of TTHL on human cell lines

	IC50 (μg/mL)^a ± SD
Time of Treatment (h)	Selectivity index (SI)^b/superscript value
Time of Treatment (h)	MRC5	MCF-7	HepG2	T24	HCT116	HT29	CACO-2
24	8.40 ± 0.28	1.36 ± 0.05^6X	6.50 ± 0.43^1.3X	5.55 ± 0.64^1.5X	7.05 ± 0.21^1X	8.00 ± 0.14^1X	8.55 ± 0.35^MR
48	7.60 ± 0.14	0.73 ± 0.05^10X	6.10 ± 0.28^1X	5.20 ± 0.42^1.5X	5.87 ± 0.04^1X	7.00 ± 0.42^1X	7.90 ± 0.28^MR
72	7.40 ± 0.15	0.63 ± 0.04^12X	5.45 ± 0.35^1X	1.09 ± 0.14^7X	5.20 ± 0.28^1X	6.15 ± 0.21^1X	6.35 ± 0.35^1X
120	6.84 ± 0.10	0.30 ± 0.04^23X	0.88 ± 0.13^8X	0.39 ± 0.10^17X	4.50 ± 0.29^1.5X	5.30 ± 0.28^1X	5.00 ± 0.19^1X
24/MXT ^c	2.88 ± 1.44	0.87 ± 1.59	3.5 ± 0.39	2.50 ± 0.62	0.61 ± 0.67	0.88 ± 2.33	2.4 ± 1.81

^aDrug concentration required to inhibit the cell growth by 50% after 24 h of incubation. ^bSelectivity index (in vitro): IC50 in MRC5 cells/IC50 in tumoral cells. Data represent mean ± three separate experiments. ^cMitoxantrone (MXT) was used as positive control. MR: more resistant in tumoral cells.

ISSN: 2662-7671