Figure 6
Flow cytometry detection of reactive oxygen species in MCF-7 cells challenged with TTHL. Cells were treated with vehicle negative control, IC20 = 0.50 μg/mL, IC50 = 1.36 μg/mL, and IC80 = 3.70 μg/mL TTHL after 24 hours’s treatment, and 500 μM H2O2. Mean data for DCF fluorescence. Results are expressed as means ± standard deviation (SD). Data were analyzed by one-way analysis of variance (ANOVA), and means were compared using Tukey test, with P ≤ 0.05 considered as statistically significant.