Item | Condition | ||
---|---|---|---|
Mobile phase | Time (min) | Water (containing 0.1% TFA) | Acetonitrile |
 | 0 | 90 | 10 |
 | 5 | 90 | 10 |
 | 40 | 10 | 90 |
 | 40 | 0 | 100 |
 | 60 | 0 | 100 |
Flow rate | 1.0Â mL/min | ||
Inject volume | 20 μL | ||
Column | OptimaPak C18 (4.6 × 250 mm, 5 μm, RS tech Co., Daejeon, Korea) | ||
Column temperature | 40°C | ||
UV wavelength | 220Â nm |