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Table 2 Effects of Polygonum viviparum (PV) on nitric oxide (NO) production by human umbilical vein endothelial cells (HUVECs)

From: Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells

 

Nitrite [μM]/5 × 105cells

 

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PV (30 μg/ml)

ACh (30 μM)

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0.378 ± 0.559

8.445 ± 0.878

7.412 ± 0.661

l-NAME (50 μM)

0.631 ± 0.518

2.073 ± 0.244**

1.066 ± 0.165##

l-NMMA (50 μM)

0.476 ± 0.483

2.232 ± 0.383**

1.154 ± 0.397##

Ca2+-free

0.505 ± 0.442

0.668 ± 0.324**

0.964 ± 0.352##

BAPTA-AM (20 μM)

0.432 ± 0.378

0.584 ± 0.460**

0.511 ± 0.369##

  1. HUVECs were incubated with PV and acetylcholine (ACh) for 1 h, and then the culture medium was collected for NO detection with the Griess reagent. Ca2+-free medium contained 2.5 mM EGTA. BAPTA-AM chelates intracellular Ca2+. Data are expressed as the mean ± standard error of the mean (S.E.M) for more than 5 individual experiments. ** p < 0.01 indicates a significant difference from PV alone. ## p < 0.01 indicates a significant difference from ACh alone.
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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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