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Figure 4 | BMC Complementary and Alternative Medicine

Figure 4

From: MiR-181a contributes to bufalin-induced apoptosis in PC-3 prostate cancer cells

Figure 4

Bufalin-induced caspase-3 activity was reduced by miR-181a inhibitor transfection in PC-3 cell. A. Caspase-3 activity was assayed by a caspase-3 substrate cleavage based luminescence kit. Histogram showed the background subtracted luminescence value. P values were calculated byStudent’s t-test, based on three replications. B. Western blot showed that the active form of caspase-3 was reduced by miR-181a inhibitor transfection. β-actin served as internal control; miR-NC indicated a negative miRNA control.

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