Effect of TD on protein expression of p53, Bcl2, Bax, cytochrome C, Caspase 9 and Caspase 3 in control and treated Huh7 cells. Evaluation of protein expression of p53 (a), Bax (b) Bcl-2 (c), cytochrome c (d), Caspase 9 (e) and Caspase 3(f). The cells were treated with TD at the concentrations 50 and 100 μg/ml for 48 h. Relative expression levels of proteins were expressed as the relative intensity of the bands. Evaluation of Caspase-3 activity in Huh7 cells by colorimetric method (g). Values are the mean ± SD of data from three independent experiments.