Figure 10

Morphological observation with DAPI staining by fluorescence microscope for SKOV-3 cells at 24 hours (magnification 400×). Arrows indicate signs of nuclear shrinkage and chromatin condensation. The untreated cells were rounded in shape and the large nuclei were homogenously stained with less bright blue color. Note that the cells were treated with the following extracts/sub-fractions: a = control, b = leaf hexane, c = leaf chloroform, d = rhizome chloroform, e = fraction LC4, f = Sub-fraction VLC9, g = fraction RC5 and h = positive control. Figures shown were obtained from at least three independent experiments with similar parameter.