Figure 5

Gross and microscopic changes in the liver of the control and high-fat diet groups with and without camel milk intake. Part I: Gross macroscopic evaluation of the liver from the Control group ( part A) and the control + camel milk Group ( part B) showed red, smooth and shiny liver tissues. Livers from the Cholesterol Group ( part C) that received the high-fat diet for eight weeks were extremely pale, enlarged, and extensively infiltrated with white spots that reflect fat accumulation inside the hepatic cells. The Cholesterol + milk group ( part D) received camel milk for eight weeks, and the livers showed marked improvement compared to the livers of the untreated group; the livers were slightly pale with few scattered white spots compared with the Ch Group. Part II: 40X photomicrograph of the H and E stained liver tissue of the Control ( part E) and the Control + milk groups ( part F) showing normal polyhedral hepatocytes with a central nucleus and eosinophilic cytoplasm. In contrast, the Ch Group ( part G) showed ballooning degeneration of the hepatocytes, loss of cytoplasmic eosin and eccentric nuclei, with diffuse microvesicular and macrovesicular steatosis, and foci of lobular inflammation and necrosis. Camel milk treatment in the ChM Group ( part H) markedly attenuated the histopathological characteristics of NASH observed in the Ch Group. The ChM group showed only mild microvesicular steatosis, no inflammatory foci, and intact architecture.