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Figure 4 | BMC Complementary and Alternative Medicine

Figure 4

From: Shikonin suppresses ERK 1/2 phosphorylation during the early stages of adipocyte differentiation in 3T3-L1 cells

Figure 4

Effects of the ERK inhibitor and activator on the inhibition of adipocyte differentiation by shikonin. (A) The protein level and mRNA expression were determined with ERK 1/2. Cells were treated with (A) Shikonin (1 μM) and the ERK inhibitor PD98059 (10 μM) during adipocyte differentiation. After 8 days, the cells were stained with Oil Red O to determine the degree of lipid accumulation. The inhibitory effects of shikonin and PD98059 on ERK 1/2 signaling pathway were observed during adipocyte differentiation. (B) Western blot analysis performed with the ERK pathway and adipogenic marker genes.

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