Rhaphidophora korthalsii modulates peripheral blood natural killer cell proliferation, cytokine secretion and cytotoxicity

Table 2 ELISA Granzyme B, TNF-α and IFN-γ detection of untreated, human rIL-2 treated, direct or indirect R. korthalsii methanol extract stimulated Human NK cells

	Granzyme B	TNF-α	IFN-γ
	(pg/mL)
CD56 NK cell
Untreated NK cells	72.3 ± 9.5ⁱ	89.1 ± 11.3^x	857.5 ± 19.5^a
Human rIL-2 treated NK cells	86.2 ± 4.1ⁱ	743.3 ± 15.6^y	3672.6 ± 28.1^b
Direct R. korthalsii extract treated NK cells	79.1 ± 8.5ⁱ	478.9 ± 17.8^z	2954.2 ± 27.9^c
Indirect R. korthalsii extract treated NK cells	88.5 ± 7.3ⁱ	814.1 ± 21.3^zz	6238.8 ± 38.2^d
Direct R. korthalsii extract treated NK cells + MQ1-17H12 antibody	78.4 ± 5.7ⁱ	442.9 ± 26.5^z	2713.9 ± 33.5^c
Indirect R. korthalsii extract treated NK cells + MQ1-17H12 antibody	83.6 ± 6.2ⁱ	527.4 ± 31.3^z	3112.2 ± 41.7^c
CD3 T cell
Untreated CD3 T cells	66.4 ± 4.7ⁱ	84.5 ± 9.3 ^x	691.2 ± 28.5^e
Human rIL-2 treated CD3 T cells	69.8 ± 5.2ⁱ	876 ± 24.7^zz	2976 ± 36.1^c
Direct R. korthalsii extract treated CD3 T cells	65.3 ± 7.1ⁱ	215.8 ± 41.2^zzz	977.2 ± 28.4^f
Indirect R. korthalsii extract treated CD3 T cells	68.2 ± 8.3ⁱ	164.5 ± 33.9^zzz	756.3 ± 23.9 ^e

Values (n = 9; 3 independent experiments with three technical replicates each) represent the means ± S.E.M. Significant (P < 0.05). i indicate no significant different among the groups for Granzyme B; x, y, z, zz, zzz indicate significant different among the groups for TNF-α while a, b, c. d. e and f indicate significant different among the groups for IFN-γ (P < 0.05).

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