Figure 4

Flow cytometry analysis of CD56 and CD16 expression of NK cells isolated from treated and non-treated PBMC by using immunomagnetic negative selection NK cell isolation kit (Milteny Biotech, Germany). Gradual increased of NK cells co-expressed the CD56^bright and CD16^dim were observed in A to C. A. Untreated NK cells, B. rIL-2 treated NK cells, C. indirect R. korthalsii methanol extract treated NK cell. Values (n = 9; 3 independent experiments with 3 technical replicates each) represent the means ± S.E.M. * Significant (P < 0.05).