Figure 3

TLBZT and 5-FU activitied Caspases. After three weeks treatment, CT26 carcinomas (3 tumors/group) were removed, homogenized, and subjected to Caspase-3, Caspase-8 and Caspase-9 activities assay by cleavage of specific substrate. Caspases activities were expressed as fold activation over control (A). Cleavage of PARP was detected by immunohistochemistry (3 tumors/group) and observed under microscope (×200) (B). Cleaved PARP positive cells (3 HPF/slide) were counted by Image-Pro Plus 6.0 software and expressed as percentage of total cells (C). D, PARP Cleavage in CT26 carcinomas were further verified with western blot by antibody against cleavage PARP (c-PARP). GAPDH was used as a loading control. **P<0.01, versus control group, ^## P<0.01, versus TLBZT or 5-Fu group.