Volume 12 Supplement 1

Scientific Abstracts Presented at the International Research Congress on Integrative Medicine and Health 2012

Open Access

P01.45. Activated neuropathway from nucleus tractus solitarius to rostal ventrolateral medulla during electroacupuncture

  • Z Guo1 and
  • J Longhurst1
BMC Complementary and Alternative MedicineThe official journal of the International Society for Complementary Medicine Research (ISCMR)201212(Suppl 1):P45

https://doi.org/10.1186/1472-6882-12-S1-P45

Published: 12 June 2012

Purpose

Our previous studies have shown that electroacupuncture (EA) at the Jianshi-Neiguan acupoints (P5-P6, overlying the median nerve) attenuates sympathoexcitatory responses through its influence on neuronal activity in the rostral ventrolateral medulla (rVLM). Nucleus tractus solitarius (NTS) receives inputs from somatic nerve stimulation. However, there is no information on the activation of NTS neurons by EA at P5-P6 acupoints, which subsequently affects the rVLM. Thus, the present study evaluated neuronal activation of NTS in response to EA, with regard to their projections to the rVLM.

Methods

Seven to ten days after unilateral microinjection of a rodamine-conjugated microsphere retrograde tracer (100 nl) into the rVLM, rats were subjected to EA or served as a sham-operated control. EA was performed for 30 min at P5-P6 acupoints bilaterally.

Results

Perikarya containing the microsphere tracer were found in the NTS of both groups. Compared to controls (needle placement without electrical stimulation, n=4), c-Fos immunoreactivity and neurons double-labeled with c-Fos, an immediate early gene and the tracer were more frequently found in the NTS of EA-treated rats (n=5), particularly, in the medial and lateral subdivisions of caudal and intermediate NTS extension.

Conclusion

These results suggest that EA at P5-P6 acupoints activates NTS neurons. Furthermore, EA-activated NTS neuron can directly project to the rVLM, which is known to participate in EA-modulation of sympathetic activity.

Authors’ Affiliations

(1)
University of California

Copyright

© Guo and Longhurst; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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